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Project Title
 Intracellular Dynamics: Chemotaxis and Cell Migration
 

Research Program
Biomolecular Dynamics
 
Project #
BD2
 

Principal Investigators
Eberhard Bodenschatz (Cornell)
Junlin Guan (Cornell)

 

Project Summary
 This program details a multidisciplinary approach between medicine, biology, and physics to use microfabrication and genetic manipulation tools to study the intracellular signaling network of eukaryotic cells, namely mammalian fibroblasts, mammalian neutrophils, and the amoebae Dictyolstelium D. A variety of strains with GFP-fused proteins like CRAC, CAR1, actin, myosin heavy chain, ABP120 (an actin binding protein), tubulin, and coronin will be used. In addition, pairs of signaling molecules fused with GFP and RFP respectively will be used for studying formation of signaling complexes in mammalian cells using FRET (fluorescence resonance energy transfer). If needed, new strains, constructs, and cell lines will be created using the highly advanced genetic engineering capabilities available in Guan's laboratory. Microfluidic incubators and patterned surfaces will be developed to apply controlled spatio-temporal stimuli. The intracellular response will be monitored using NBTC's confocal microscopy system, combined with brightfield fluorescence imaging and image processing available in both research groups.

 

This material is based upon work supported in part by the STC Program of the National Science Foundation under Agreement No. ECS-9876771. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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